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Abstract Human intestinal organoids (HIOs) are vital for modeling intestinal development, disease, and therapeutic tissue regeneration. However, their susceptibility to stress, immunological attack, and environmental fluctuations limits their utility in research and therapeutic applications. This study evaluated the effectiveness of temporary silk protein‐based layer‐by‐layer (LbL) nanoencapsulation technique to enhance the viability and functions of HIOs against common biomedical stressors, without compromising their native functions. Cell viability and differentiation capacity are assessed, finding that nanoencapsulation significantly improved HIO survival under the various environmental perturbations studied without compromising cellular functionality. Post‐stress exposures, the encapsulated HIOs still successfully differentiated into essential intestinal cell types such as enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Moreover, the silk nanocoatings effectively protected against environmental stressors such as ultraviolet (UV) light exposure, protease degradation, antibody binding, and cytokine‐induced inflammation. This nanoencapsulation technique shows promise for advancing HIO applications in disease modeling, drug testing, and potential transplantation therapies. 

Encapsulation of single cells is a powerful technique used in various fields, such as regenerative medicine, drug delivery, tissue regeneration, cell-based therapies, and biotechnology. It offers a method to protect cells by providing cytocompatible coatings to strengthen cells against mechanical and environmental perturbations. Silk fibroin, derived from the silkworm Bombyx mori, is a promising protein biomaterial for cell encapsulation due to the cytocompatibility and capacity to maintain cell functionality. Here, THP-1 cells, a human leukemia monocytic cell line, were encapsulated with chemically modified silk polyelectrolytes through electrostatic layer-by-layer deposition. The effectiveness of the silk nanocoating was assessed using scanning electron microscopy (SEM) and confocal microscopy and on cell viability and proliferation by Alamar Blue assay and live/dead staining. An analysis of the mechanical properties of the encapsulated cells was conducted using atomic force microscopy (AFM) nanoindentation to measure elasticity maps and cellular stiffness. After the cells were encapsulated in silk, an increase in their stiffness was observed. Based on this observation, we developed a mechanical predictive model to estimate the variations in stiffness in relation to the thickness of the coating. By tuning the cellular assembly and biomechanics, these encapsulations promote systems that protect cells during biomaterial deposition or processing in general. 

Abstract We present a systematic search for tidal disruption events (TDEs) using radio data from the Variables and Slow Transients (VAST) Pilot Survey conducted using the Australian Square Kilometre Array Pathfinder. Historically, TDEs have been identified using observations at X-ray, optical, and ultraviolet wavelengths. After discovery, a few dozen TDEs have been shown to have radio counterparts through follow-up observations. With systematic time-domain radio surveys becoming available, we can now identify new TDEs in the radio regime. A population of radio-discovered TDEs has the potential to provide several key insights including an independent constraint on their volumetric rate. We conducted a search to select variable radio sources with a single prominent radio flare and a position consistent within 2σof the nucleus of a known galaxy. While TDEs were the primary target of our search, sources identified in this search may also be consistent with active galactic nuclei exhibiting unusual flux density changes at the timescales probed, uncharacteristically bright supernovae, or a population of gamma-ray bursts. We identify a sample of 12 radio-bright candidate TDEs. The timescales and luminosities range from ∼6 to 230 days and ∼10³⁸to 10⁴¹erg s⁻¹, respectively, consistent with models of radio emission from TDEs that launch relativistic jets. After calculating the detection efficiency of our search using a Monte Carlo simulation of TDEs, and assuming all 12 sources are jetted TDEs, we derive a volumetric rate for jetted TDEs of ${0.80}_{-0.23}^{+0.31}$Gpc⁻³yr⁻¹, consistent with previous empirically estimated rates. 

ABSTRACT We present our analysis of supernovae serendipitously found to be radio-bright several years after their optical discovery. We used recent observations from the Australian SKA Pathfinder (ASKAP) taken as part of the pilot Variables and Slow Transients and Rapid ASKAP Continuum Survey programmes. We identified 29 objects by cross-matching sources from these ASKAP observations with known core-collapse supernovae below a declination of $$+40^{\circ }$$ and with a redshift of $$z\le 0.15$$. Our results focus on eight cases that show potential late-time radio emission. These supernovae exhibit significantly greater amounts of radio emission than expected from the standard model of a single shockwave propagating through a spherical circumstellar medium, with a constant density structure produced by regular stellar mass-loss. We also discuss how we can learn from future ASKAP surveys about the circumstellar environments and emission mechanisms of supernovae that undergo late-time radio re-brightening. This pilot work tested and confirmed the potential of the Variables and Slow Transients survey to discover and study late-time supernova emission. 

ABSTRACT The population of radio-loud stars has to date been studied primarily through either targeted observations of a small number of highly active stars or wide-field, single-epoch surveys that cannot easily distinguish stellar emission from background extragalactic sources. As a result it has been difficult to constrain population statistics such as the surface density and fraction of the population producing radio emission in a particular variable or spectral class. In this paper, we present a sample of 36 radio stars detected in a circular polarization search of the multi-epoch Variables and Slow Transients (VAST) pilot survey with ASKAP at 887.5 MHz. Through repeat sampling of the VAST pilot survey footprint we find an upper limit to the duty cycle of M-dwarf radio bursts of $$8.5 \,\rm {per\,cent}$$, and that at least 10 ± 3 $$\rm {per\,cent}$$ of the population should produce radio bursts more luminous than $$10^{15} \,\rm {erg}\mathrm{s}^{-1} \,\mathrm{Hz}^{-1}$$. We infer a lower limit on the long-term surface density of such bursts in a shallow $$1.25 \,\mathrm{m}\rm {Jy}\rm\ {PSF}^{-1}$$ sensitivity survey of $${9}^{\, +{11}}_{-{7}}\times 10^{-3}$$  $$\,\deg ^{-2}$$ and an instantaneous radio star surface density of 1.7 ± 0.2 × 10−3  $$\,\deg ^{-2}$$ on 12 min time-scales. Based on these rates we anticipate ∼200 ± 50 new radio star detections per year over the full VAST survey and $${41\, 000}^{\, +{10\, 000}}_{-{9\, 000}}$$ in next-generation all-sky surveys with the Square Kilometre Array. 

Abstract We report on the results of an image-based search for pulsar candidates toward the Galactic bulge. We used mosaic images from the MeerKAT radio telescope that were taken as part of a 173 deg²survey of the bulge and Galactic center of our Galaxy atLband (856–1712 MHz) in all four StokesI,Q,U, andV. The image rms noise levels of 12–17μJy ba⁻¹represent a significant increase in sensitivity over past image-based pulsar searches. Our primary search criterion was circular polarization, but we used other criteria, including linear polarization, in-band spectral index, compactness, variability, and multiwavelength counterparts to select pulsar candidates. We first demonstrate the efficacy of this technique by searching for polarized emission from known pulsars and comparing our results with measurements from the literature. Our search resulted in a sample of 75 polarized sources. Bright stars or young stellar objects were associated with 28 of these sources, including a small sample of highly polarized dwarf stars with pulsar-like steep spectra. Comparing the properties of this sample with the known pulsars, we identified 30 compelling candidates for pulsation follow-up, including two sources with both strong circular and linear polarization. The remaining 17 sources are either pulsars or stars, but we cannot rule out an extragalactic origin or image artifacts among the brighter, flat-spectrum objects. 

Abstract We present an intensive multiwavelength monitoring campaign of the quasar PG 1302−102 with Swift and the Las Cumbres Observatory network telescopes. Atz∼ 0.3, it tests the limits of the reverberation mapping (RM) technique in probing the accretion disk around a supermassive black hole (SMBH) and extends the parameter space to high masses and high accretion rates. This is also the first time the RM technique has been applied to test disk structures predicted in the SMBH binary model that has been suggested for this source. PG 1302−102 was observed at a ∼daily cadence for ∼9 months in 14 bands spanning from X-ray to UV and optical wavelengths, and it shows moderate to significant levels of variability correlated between wavelengths. We measure the interband time lags, which are consistent with aτ∝λ^4/3relation as expected from standard disk reprocessing, albeit with large uncertainties. The disk size implied by the lag spectrum is consistent with the expected disk size for its black hole mass within uncertainties. While the source resembles other reverberation-mapped active galactic nuclei in many respects, and we do not find evidence supporting the prevalent hypothesis that it hosts an SMBH binary, we demonstrate the feasibility of studying SMBH binaries from this novel angle and suggest possibilities for the LSST Deep Drilling Fields. 

Abstract We report the discovery of a young, highly scattered pulsar in a search for highly circularly polarized radio sources as part of the Australian Square Kilometre Array Pathfinder Variables and Slow Transients survey. In follow-up observations with the Parkes radio telescope, Murriyang, we identified PSR J1032−5804 and measured a period of 78.7 ms, a dispersion measure of 819 ± 4 pc cm⁻³, a rotation measure of −2000 ± 1 rad m⁻², and a characteristic age of 34.6 kyr. We found a pulse scattering timescale at 3 GHz of ∼22 ms, implying a timescale at 1 GHz of ∼3845 ms, which is the third most scattered pulsar known and explains its nondetection in previous pulsar surveys. We discuss the identification of a possible pulsar wind nebula and supernova remnant in the pulsar’s local environment by analyzing the pulsar spectral energy distribution and the surrounding extended emission from multiwavelength images. Our result highlights the possibility of identifying extremely scattered pulsars from radio continuum images. Ongoing and future large-scale radio continuum surveys will offer us an unprecedented opportunity to find more extreme pulsars (e.g., highly scattered, highly intermittent, and highly accelerated), which will enhance our understanding of the characteristics of pulsars and the interstellar medium. 

Abstract Although neutron star–black hole binaries have been identified through mergers detected in gravitational waves, a pulsar–black hole binary has yet to be detected. While short-period binaries are detectable due to a clear signal in the pulsar’s timing residuals, effects from a long-period binary could be masked by other timing effects, allowing them to go undetected. In particular, a long-period binary measured over a small subset of its orbital period could manifest via time derivatives of the spin frequency incompatible with isolated pulsar properties. We assess the possibility of pulsars having unknown companions in long-period binaries and put constraints on the range of binary properties that may remain undetected in current data, but that may be detectable with further observations. We find that for 35% of canonical pulsars with published higher-order derivatives, the precision of measurements is not enough to confidently reject binarity (period ≳2 kyr), and that a black hole binary companion could not be ruled out for a sample of pulsars without published constraints if the period is >1 kyr. While we find no convincing cases in the literature, we put more stringent limits on orbital period and longitude of periastron for the few pulsars with published higher-order frequency derivatives (n≥ 3). We discuss the detectability of candidates and find that a sample pulsar in a 100 yr orbit could be detectable within 5–10 yr.